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Nucleic Acids Symposium Series 2009 53(1):29-30; doi:10.1093/nass/nrp015
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© 2009 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: The 6th International Symposium on Nucleic Acids Chemistry (36th Symposium on Nucleic Acids Chemistry) [View the issue table of contents]

Efficient energy transfer from pyrene to perylene assembled inside DNA duplex

Hiromu Kashida1, Tomohiko Takatsu1 and Hiroyuki Asanuma1,2,*

1Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan and 2CREST, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

*Corresponding author. E-mail: asanuma{at}mol.nagoya-u.ac.jp.

Abstract

Pyrene (donor) and perylene (acceptor) were assembled in a DNA duplex to increase the apparent Stokes’ shift of perylene. Multiple donors were introduced in the vicinity of acceptors via D-threoninol and natural base pairs were inserted between donors and acceptors in order to suppress undesired interactions between them. When two pyrene moieties were located in proximity to one perylene with one base pair inserted between them, efficient FRET occurred within the duplex. Thus, strong emission at 495 nm was observed from perylene when excited at 345 nm where pyrene has its absorption. The apparent Stokes’ shift became as large as 115 nm with a high FRET efficiency ({Phi}>1). However, the introduction of more than two pyrenes did not enhance the fluorescence intensity of perylene, due to the short Förster radius (R0) of the donor pyrene.


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