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Nucleic Acids Symposium Series 2009 53(1):47-48; doi:10.1093/nass/nrp024
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© 2009 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: The 6th International Symposium on Nucleic Acids Chemistry (36th Symposium on Nucleic Acids Chemistry) [View the issue table of contents]

Assessment of the DNA damage using the fluorescence microscope

Yuka Yamazaki, Anatoly Zinchenko and Shizuaki Murata*

Graduate School of Environmental Studies, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

*Corresponding author. E-mail: murata{at}urban.env.nagoya-u.ac.jp.

Abstract

DNA damage was monitored by fluorescent microscopy observations of DNA fluorescent images after hydrodynamic stretching on a microscope glass. DNA double-strand breaks lead to a decrease of the average length of observed fluorescent DNA molecules. Compared to conventional methods such as electrophoresis, the proposed method allows for the analysis of the DNA damage at very low DNA breaking frequency. In particular, this method was used to study DNA damage by weak UV irradiation in solutions of quantum dots.


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