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Nucleic Acids Symposium Series 2001 1(1):21-22; doi:10.1093/nass/1.1.21
© 2001 by Oxford University Press
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Enzymatic synthesis of modified DNA by PCR

Mohammad Mehedi Masud, Akiko Ozaki-Nakamura, Fumie Satou, Tsutomu Ohbayashi, Hiroaki Ozaki and Hiroaki Sawai

Department of Applied Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan

We synthesized various 5'-triphosphates of C5-substituted 2'-deoxyuridine derivatives bearing methylene linker at CS-{alpha} position. We examined whether the C5-substituted 2'-deoxyuridine 5'-triphosphates (dUTP) can work as a substrate for the modified DNA synthesis by PCR. We found that only KOD dash DNA polymerase, a thermostable DNA polymerase from extremely thermophilic archaeum, accepted the modified substrates in place of TTP for PCR forming the corresponding modified DNAs. On the other hand, no other DNA polymerase could accept these TTP analogues.


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