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Nucleic Acids Symposium Series 2001 1(1):37-38; doi:10.1093/nass/1.1.37
© 2001 by Oxford University Press
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Development of ribozyme synthesis system using a rolling- synchronization: Effect of template DNA secondary structure on recognition of RNA polymerase

Tatsuo Ohmichi1, Megumi Nakamura2 and Naoki Sugimoto1,2

1 High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan, 2 Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan

Circular DNA behaves as an efficient template for RNA polymerase (RNAP) in rolling circle transcription (RCT). We found that the short hairpin-stem structure in the circular DNA without promoter enhances the DNA recognition of T7 RNAP in RCT. The result indicates that T7 RNAP would distinguish the short DNA hairpin-stem structure.


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