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Nucleic Acids Symposium Series 2001 1(1):45-46; doi:10.1093/nass/1.1.45
© 2001 by Oxford University Press
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Quantitation of DNA damage by an aldehyde reactive probe (ARP)

Satofumi Kurisu1, Toshinobu Miya1, Hiroaki Terato1, Aya Masaoka1, Yoshihiko Ohyama1, Kihei Kubo2 and Hiroshi Ide1

1 Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan, 2 Division of Veterinary Science, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan

DNA is constantly damaged by endogenous and environmental agents. Abasic sites representing a major class of DNA damage can be quantitated by an ELISA-like assay using an aldehyde reactive probe (ARP). It is shown that oxidative pyrimidine lesions can be also quantitated by the ARP assay in combination with the treatment with endonuclease III.


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