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Nucleic Acids Symposium Series 2001 1(1):67-68; doi:10.1093/nass/1.1.67
© 2001 by Oxford University Press
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Regulatory mechanism of the gene expression during chlorovirus infection cycle

Takeru Kawasaki, Masahiro Tanaka, Kensho Nishida and Takashi Yamada

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan

In chlorovirus infection, immediate-early gene expression occurs within 5–20 min post infection (p.i.). We identified 16 major immediate-early transcripts. On Northern blot analysis, these transcripts showed unusual behaviors: (I) after 40 min p.i., their sizes reduced by approximately 100 nt, most likely caused by loss of a poly(A) tail and (II) after 40 min p.i., often appeared a few larger additional transcripts that may be formed by read-through from an upstream gene or into a downstream gene. These results indicate that the transcription of the viral genes changes drastically between before and after 40 min p.i.


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