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Nucleic Acids Symposium Series 2001 1(1):77-78; doi:10.1093/nass/1.1.77
© 2001 by Oxford University Press
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A new coupling method for the synthesis of oligodeoxyribonucleotides based on protection by protonation of nucleoside bases

Akihiro Ohkubo, Kohji Seio and Mitsuo Sekine

Department of life science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan

We developed a conceptually new strategy for the coupling method in the phosphoramidite method without protection of nucleoside bases. This strategy, called "proton-block method", significantly reduced side reactions compared with the previous methods.


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