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Nucleic Acids Symposium Series 2002 2(1):113-114; doi:10.1093/nass/2.1.113
© 2002 by Oxford University Press
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Development and application of siRNA expression vector

Makoto Miyagishi1,2 and Kazunari Taira1,2

1 Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan, 2 Gene Function Research Lab., National Institute of Advanced Industrial Science and Technology (NIAIST), 1-1-4 Higashi, Tsukuba Science City 305-8562, Japan

RNA interference (RNAi) is a sequence-specific silencing phenomenon, which is induced by double-stranded RNA (dsRNA) and mediated through an evolutionary conserved mechanism from plants to mammals. In mammalian cells, it has recently been reported that 21- or 22-nucleotide (nt) RNAs with 2-nt 3' overhangs (siRNA) induce RNAi without induction of the dsRNA-dependent inhibition of protein synthesis, known as the host defense system against viral infections. Moreover, we and other have developed siRNA expression systems utilizing a pol III promoter. Here we report a comparative analysis among various siRNA expression vectors and also demonstrate a regulatable RNAi in cells by using a tetracycline-controlled U6 promoter.


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