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Nucleic Acids Symposium Series 2002 2(1):117-118; doi:10.1093/nass/2.1.117
© 2002 by Oxford University Press
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Synthesis and application of bis (tert-butyl)-1-methyl pyrenyl-silyl chloride as a 5'-hydroxyl protecting group for nucleosides and as a fluorescent purification handle in oligonucleotide synthesis

Snehlata Tripathi1, Krishna Misra1 and Yogesh S. Sanghvi2

1 Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad-211002, India, 2 lsis Pharmaceuticals USA, Carlsbad Research Center, 2292 Faraday Avenue, Carlsbad, CA 92008

A fluorescently labelled monomer viz. 5'-O-1, 1-bis- (tert-butyl) -1'-methyl -pyrenyl -silyl -thymidine -3'-phosphoramidite was synthesized. Different sequences were synthesized and were labelled using it and their fluorescence studies were carried out.


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