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Nucleic Acids Symposium Series 2003 3(1):47-48; doi:10.1093/nass/3.1.47
© 2003 by Oxford University Press
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Stretching of single DNA molecules by LB technique for restriction site mapping

Kuniharu Ijiro1,2, Yasutaka Matsuo1 and Masatsugu Shimomura3

1 PRESTO, Japan Science and Technology Corporation, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan, 2 Research Institute for Electronic Science, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan, 3 Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan

Stretched single DNA molecules were prepared on substrates by Langmuir-Blodgett (LB) method for the restriction site mapping. Stretched single Lambda DNA and EcoRI bound to the DNA were observed by scanning near-field optical microscope (SNOM). As a result, the specific binding sites of EcoRI on the Lambda DNA molecules were determined.


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