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Nucleic Acids Symposium Series 2000 44(1):187-188; doi:10.1093/nass/44.1.187
© 2000 by Oxford University Press
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In vitro selection and analysis of RNA aptamer recognize arginine-rich motif (ARM) model peptide on a QCM

Shinobu Fukusho, Hiroyuki Furusawa and Yoshio Okahata

Department of Biomolecular Engineering, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

To study RNA-peptide interactions, we performed an in vitro selection of RNA on a 27 MHz quartz-crystal microbalance (QCM) on which a simple R5 helix peptide was immobilized as a model of N peptide from bacteriophade {lambda}. The consensus sequences including a GNRA tetraloop were obtained from a random RNA pool after the 7th cycle selection.


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