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Nucleic Acids Symposium Series 2004 48(1):199-200; doi:10.1093/nass/48.1.199
© 2004 by Oxford University Press
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Photoregulation of in vitro transcription/translation of GFP by tethering an azobenzene to T7 promoter

Jing Zhao2, Jingmin Zhou1, Mingzhe Liu1, Hiroyuki Asanuma1,2 and Makoto Komiyama1

1 Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan, 2 Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Corporation (JST), Kawaguchi 332-0012, Japan

Based on a previous study of photo-regulation of transcription by T7 RNA polymerase, the regulatory effect of azobenzene on in vitro green fluorescent protein (GFP) production was studied by introduction of a D-threoninol-tethered p-azobenzene into the non template strand of the T7 promoter in a GFP template By measurement of the resulting GFP fluorescence intinsih after the in vitro transcription/translation coupled reaction, it is found thai photo-regulation of GFP gene expression can be achieved in a position-dependent manner by trans-cis isomerization of azobenzene upon UV irradiation.


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