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Nucleic Acids Symposium Series 2004 48(1):203-204; doi:10.1093/nass/48.1.203
© 2004 by Oxford University Press
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Analysis of the secondary structure of a ribozyme that requires His as a cofactor

Junji Kawakami1,2, Chisako Maiya1 and Naoki Sugimoto1,2

1 Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan, 2 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan

A novel ribozyme that requires L-histidine for expression of the function was revealed from an in vitro selection experiment. Here, we report the secondary structural analysis of the ribozyme. Several deletion and point mutants were designed based on the possible secondary structures predicted by a thermodynamic method with nearest neighbor parameters. As a result, formation of two important stems was essential for folding into the active structure.


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