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Nucleic Acids Symposium Series 2004 48(1):217-218; doi:10.1093/nass/48.1.217
© 2004 by Oxford University Press
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Synthesis and hybridization studies on oligonucleotide-metal complex conjugates

Kazuhiro Hara, Masaya Kitamura and Hideo Inoue

Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585,Japan

We synthesized a DNA oligomer with a terpyridine·Cu(II) complex-linked thymidine residue at phosphors the 5'-end. The attachment of the 5'-complex slightly enhanced the thermal stability of the DNA match-duplex. We also determined the thermal stabilities of the modified DNA duplexes and their unmodified counterparts, with a mismatched base-pair, to examine the effect of the complex on the stability.


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