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Nucleic Acids Symposium Series 2004 48(1):65-66; doi:10.1093/nass/48.1.65
© 2004 by Oxford University Press
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Synthesis and base recognition ability of oligoribonucleotides incorporating 2'-O-methyl-3-deazaguanosine

Kohji Seio1,3, Takeshi Sasami2 and Mitsuo Sekine2,3

1 Frontier Collaborative Research Center, Tokyo Institute of Technology, Japan, 2 Department of Life Science, Tokyo Institute of Technology, Japan, 3 CREST, JST (Japan Science and Technology Corporation), Nagatsuda, Midoriku, Yokohama 226-8503, Japan

2'-O-Methyl-Oligoribonucleotides incorporating a 3-deazaguanine (3DG) were synthesized. Melting temperature (Tm value) analysis indicates the stability and base discriminating ability of the 2'-O-methl-RNA/RNA duplex.


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