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Nucleic Acids Symposium Series 2005 49(1):19-20; doi:10.1093/nass/49.1.19
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© 2005 Oxford University Press

SNPs analysis by use of protected oligonucleotide probes

Akihiro Ohkubo1,3, Rintarou Kasuya1, Kazushi Sakamoto, Haruhiko Taguchi1,3, Kohji Seio2,3 and Mitsuo Sekine1,3

1 Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8501, Japan, 2 Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8503, Japan, 3 CREST, JST

Loss of large amounts of DNA probe occurred at the deprotection step of the in situ synthesis of DNA microarrays for analysis of SNPs or gene expression. In this study, we developed "Protected Oligonucleotide Probes" that can avoid elimination of probe DNAs and allow their highly efficient synthesis. The protected oligonucleotide probes containing dCac and d[oxoAac] instead of dC and dA proved to have similar affinity for ssDNA having the complementary sequence and base recognition ability compared with the corresponding unmodified DNA probes.


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