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Nucleic Acids Symposium Series 2005 49(1):201-202; doi:10.1093/nass/49.1.201
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© 2005 Oxford University Press

SNPs typing by base-discriminating fluorescence DNA probe

Akimitsu Okamoto1, Kazuki Tainaka1, Yuji Ochi1 and Isao Saito2,3

1 Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan, 2 School of Engineering, NEWCAT Institute, Nihon University, Tamura, Koriyama 963-8642, Japan, 3 SORST, Japan Science and Technology Agency, Tamura, Koriyama 963-8642, Japan

For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present SNP typing method with BDF probes is a very powerful homogeneous assay that does not require a special device or time-consuming steps.


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