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Nucleic Acids Symposium Series 2005 49(1):269-270; doi:10.1093/nass/49.1.269
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© 2005 Oxford University Press

In vitro selection of RNA aptamers for the Escherichia coli release factor 1

Atsushi Ogawa, Teruyuki Nishi, Shinsuke Sando and Yasuhiro Aoyama

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

We carried out an in vitro selection of RNA aptamers that bind to Escherichia coli release factor 1 (E. coli RF1). The selected aptamer (class II) showed an apparent dissociation constant of nM range. The binding of the class II aptamer with E. coli RF1 is highly specific (orthogonal), allowing selective inhibition of RF1 activity in the E. coli translation system.


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