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Nucleic Acids Symposium Series 2005 49(1):271-272; doi:10.1093/nass/49.1.271
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© 2005 Oxford University Press

Highly sensitive genotyping using artificial riboregulator system

Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando and Yasuhiro Aoyama

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto university, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

A molecular-beacon-type riboregulator (mRNA) was applied to multiply catalytic gene sensing. It consists of a reporter gene for firefly protein luciferase and, upstream thereof, a regulator hairpin domain composed of an RBS/anti-RBS stem (RBS = ribosome binding site) and a loop which is complementary to the target. The sensing of target gene, using an unmodified RNA or even dsDNA as a probe with a chemiluminescence output, was demonstrated with a sensitivity at ≤50 fmol of the target and a single nucleotide resolution.


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