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Nucleic Acids Symposium Series 2005 49(1):275-276; doi:10.1093/nass/49.1.275
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© 2005 Oxford University Press

Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates

Masayasu Kuwahara1,2, Takehiro Tamura1, Rina Kitagata1, Hiroaki Sawai1 and Hiroaki Ozaki1

1 Department of Applied Chemistry, Fuclty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan, 2 PRESTO (JST)

A series of C5 substituted analogs of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate were synthesized and investigated their substrate properties for thermostable DNA polymerases during polymerase chain reaction (PCR). The PCR assays indicated that the relative yield of the full-length product is dependent on whether the substitution is on dU or dC, and on the nature of the polymerase.


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