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Nucleic Acids Symposium Series 2005 49(1):3-4; doi:10.1093/nass/49.1.3
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© 2005 Oxford University Press

Properties and EPR studies of oligodeoxynucleotides containing 2-N-tert-butylaminoxylpurines

Mariko Aso, Toshiyuki Kaneko, John Walter Mirc, Noboru Koga and Hiroshi Suemune

Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.


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