Skip Navigation

Nucleic Acids Symposium Series 2005 49(1):303-304; doi:10.1093/nass/49.1.303
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Awai, T.
Right arrow Articles by Hori, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Awai, T.
Right arrow Articles by Hori, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 Oxford University Press

Aquifex aeolicus Trm1[tRNA (m22G26) methyltransferase] has a novel recognition mechanism of the substrate RNA

Takako Awai1, Toru Takehara1, Hiroshi Takeda1 and Hiroyuki Hori1,2

1 Department of Applied Chemistry, Faculty of Engineering, Ehime University, Bunkyo 3, Matsuyama 790-8577, Japan, 2 Venture Business Laboratory, Ehime University, Matsuyama 790-8577, Japan

Aquifex aeolicus is a hyper-thermophilic eubacterium, which probably diverged at the earliest period from the other bacteria on the evolution of life. Therefore, analyses of RNA modification enzymes of this bacterium may supply important information in relation to the establishment of the early protein synthesis. In the previous meeting, we have reported that Aquifex aeolicus trm1 gene product possesses a tRNA (m22G26) methyltransferase activity. This enzyme catalyzes the methyl-transfer from S-adenosyl-L-methionine to the semi-conserved residue, G26, via the intermediate modified base, m2G26. In this meeting, we report the A. aeolicus Trm1 large scale expression system in E.coli and the substrate RNA recognition mechanism of the enzyme.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.