Skip Navigation

Nucleic Acids Symposium Series 2005 49(1):33-34; doi:10.1093/nass/49.1.33
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hirao, I.
Right arrow Articles by Yokoyama, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirao, I.
Right arrow Articles by Yokoyama, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 Oxford University Press

Non-hydrogen-bonded base pairs for specific transcription

Ichiro Hirao1,2, Tsuneo Mitsui1, Michiko Kimoto2, Rie Kawai2, Akira Sato2 and Shigeyuki Yokoyama2,3,4

1 Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan, 2 Protein Reserch Group, RIKEN Genomic Sciences Center (GSC), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan, 3 Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, 4 RIKEN Harima Institute at SPring-8, 1-1-1 Kohto, Mikazuki-cho, Sayo, Hyogo 679-5148, Japan

Specific transcription mediated by unnatural base pairs could create novel RNA molecules with increased functionality and expand the genetic code. Here, we report an unnatural base pair between pyrrole-2-carbaldehyde (Pa) and 2-amino-6-(2-thienyl)purine (s) or 6-(2-thienyl)purine (s') for the site-specific incorporation of s or s' into RNA by T7 RNA polymerase, using DNA templates containing Pa. Despite the absence of significant hydrogen bonding interactions between the unnatural bases, the efficiency and fidelity of the s-Pa pairing in transcription were as high as those of the natural base pairings. As shown in replication, this indicates the importance of shape complementarity between pairing bases in transcription. Since the s base is fluorescent, this transcription mediated by the s-Pa pair provides a useful tool for site-specific fluorescence probing of RNA molecules.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.