Skip Navigation

Nucleic Acids Symposium Series 2005 49(1):69-70; doi:10.1093/nass/49.1.69
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Matsugami, A.
Right arrow Articles by Katahira, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsugami, A.
Right arrow Articles by Katahira, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 Oxford University Press

Structure of RNA aptamer complexed with an RNA-binding peptide of Tat with aid of residue-specific 13C, 15N labeling

Akimasa Matsugami1, Hidehito Tochio1, Emi Niyada1, Yusuke Tamura1, Michiko Kudo1, Tomoko S. Misono2, Penmetcha Kumar2 and Masato Katahira1,3

1 Supramolecular Biology, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan, 2 Functional Nucleic Acids Group, AIST, Central 6, 1-1 Higashi, Tsukuba 305-8566, Japan, 3 Genomic Science Center, RIKEN

An RNA aptamer containing two binding sites of HIV Tat exhibits extremely high affinity to Tat. We have determined the structure of the aptamer complexed with an RNA-binding peptide of Tat. The analysis was made feasible by the use of several peptides in which a single arginine residue was specifically 13C, 15N-labeled. Residue specific labeling of the peptide enhanced the identification of intermolecular contacts, which are otherwise hard to identify due to spectral overlapping. The structure of the complex has revealed the origin of the high affinity of the aptamer to Tat.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.