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Nucleic Acids Symposium Series 2006 50(1):171-172; doi:10.1093/nass/nrl085
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© 2006 Oxford University Press

Synthesis and application of new amine-modified oligonucleotides using H-phosphonate chemistry

Nagendra Kumar Kamisetty1, Seung Pil Pack1,2, Mitsuru Nonogawa1, Kamakshaiah Charyulu Devarayapalli1, Tsutomu Kodaki1,2 and Keisuke Makino1,2,3

1 Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan, 2 CREST, JST (Japan Science and Technology Agency), Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan, 3 International Innovation Center, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan

Oligodeoxynucleotide probes tethered with amine linkers are commonly used for development of DNA based biosensor tools for studying gene expression and biological assays. However, the current preparation methods for synthesizing amine tethered probes often show defects during acid deprotection and during hydrophobic purification with the amine linker protecting groups. Here, we developed a prospective preparation method of using modified H-phosphonate chemistry to prepare amine tethered oligonucleotide probes. The present developed method will be helpful for preparing efficient amine tethered oligodeoxynucleotide probes that can be used in DNA biosensor applications.


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