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Nucleic Acids Symposium Series 2006 50(1):199-200; doi:10.1093/nass/nrl099
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© 2006 Oxford University Press

Spectroscopic analysis of AgI-DNA complex

Shuji Oda1, Tomoyuki Komuro1, Yoshinori Kondo1, Akira Ono2 and Yoshiyuki Tanaka1

1 Graduate School of Pharmaceutical Sciences Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan, 2 Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, Yokohama 221-8686, Japan

It has recently been reoprted that C-C mismatches were able to recognize AgI specifically, and form AgI-mediated cytosine-cytosine base pairs (C-AgI-C pair). In this study, we explored DNA sequences, which are appropriate for structural studies of this new type of metal-mediated base pairs. For this experiments, we synthesized four kinds of DNA duplexes including a single C-C mismatch. By using UV spectra, we performed titration experiments of DNA duplexes with AgNO3. Within the sequences we tested, it was found that the DNA duplex: 5'-CGCGCGTCC-3' • 5'-GGACCCGCG-3' showed 1:1 complexation of this DNA duplex and AgI. In addition, a DNA duplex: 5'-TAAATCTAATA-3' • 5'-TATTACATTTA-3' also exhibited a similar character. Therefore, these two DNA duplexes are interesting candidates for structural analyses.


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