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Nucleic Acids Symposium Series 2006 50(1):261-262; doi:10.1093/nass/nrl130
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© 2006 Oxford University Press

Suppression of RUNX1 by siRNA in megakaryocytic UT-7/GM cells

Yoshiaki Okada1, Ryohei Nagai1, Eri Matsuura1, Yusuke Hoshika1, Emi Nakata1, Hironori Nagura1, Ayako Watanabe1, Norio Komatsu2 and Takefumi Doi1

1 Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, Japan, 2 Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, Japan

RUNX1 is a transcription factor that plays critical roles in hematopoietic proliferation and differentiation. Megakaryocyte is a precursor cell of platelets. Several reports have implied that RUNX1 is important in megakaryocytic differentiation and proliferation. However, the mechanism is not well understood. In this study, we employed a megakaryocytic cell line UT-7/GM and suppressed the RUNX1 gene expression by siRNA. Knocking down of RUNX1 induced the increase of megakaryocyte-specific gene expression and down-regulation of polyploidization. RUNX1 overexpression decreased the PF4 and GPIIb promoter activities. These results suggest that RUNX1 promotes proliferation of megakaryocytic cell line but not megakaryocytic gene expression in UT-7/GM cells.


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