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Nucleic Acids Symposium Series 2006 50(1):31-32; doi:10.1093/nass/nrl016
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© 2006 Oxford University Press

Substrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases

Masayasu Kuwahara1,2, Yoshiyuki Suto1, Satoshi Minezaki1, Rina Kitagata1, Jun-ichi Nagashima1,2 and Hiroaki Sawai1

1 Department of Applied Chemistry, Faculty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan, 2 PRESTO (JST)

DNA aptamers and DNAzymes with similar function to antibodies and enzymes can be produced by in vitro selection. They would be useful as research tools for molecular biology and as indicators of specific substances for the analysis of clinical and food samples. Furthermore, development of modified DNA molecules aimed to diversify function and improve activity has recently proceeded by introducing functionalities to these DNA molecules. Such functional modified DNA molecules with an aimed activity screened from a random sequence pool of modified DNA prepared by a polymerase reaction. To enhance potential of selection library and expand diversity of modified DNA that can be synthesized enzymatically, modified analogs of 2'-deoxyadenosine triphosphate were synthesized and their substrate properties for some thermostable DNA polymerases in polymerase chain reactions (PCR) were investigated. Modified DNAs were sequenced in order to analyze incorporation accuracy of modified dATP during PCR.


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