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Nucleic Acids Symposium Series 2007 51(1):21-22; doi:10.1093/nass/nrm011
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© 2007 Oxford University Press

Synthesis of Peptide Ribonucleic Acid (PRNA)-DNA Chimera and Interaction with DNA and RNA

Takehiko Wada1,2,3,*, Yoshiki Maeda2, Nobuya Sawa2, Hirofumi Sato3, Hyongi Chon2, Shigenori Kanaya2 and Yoshihisa Inoue2,4

1Institute of Multidisciplinary Research for Advanced Materials,Tohoku University, 2-1-1, Katahira, Aobaku, Sendai 980-8577, 2Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan, 3PRESTO, JST, Yamada-oka, Suita 565-0871, Japan, and 4ICORP Entropy Control Project, JST, 4-6-3 Kamishinden, Toyonaka 565-0085, Japan

*Corresponding author. hiko{at}tagen.tohoku.ac.jp

Abstract

Recently, we have demonstrated that effective control of the recognition behavior of peptide ribonucleic acid (PRNA) with complementary DNA is possible through the anti-to-synorientational change of pyrimidine nucleobase induced by borate ester formation. In this study, DNA-PRNA chimera was prepared by the solidphase synthesis. In the DNA-PRNA chimeras, both PRNA and DNA domains work as recognition sites for the complementary DNA/RNAs to form stable complex, while DNA-RNA hybrids formed in the DNA domains of DNA-PRNA chimera should be substrates to the hydrolysis by RNase H and PRNA moieties work as recognition control/switching devices and as inhibitor for the hydrolysis by exonucleases. Interaction of the DNA-PRNA chimera with DNA and RNA has been discussed.


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