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Nucleic Acids Symposium Series 2007 51(1):7-8; doi:10.1093/nass/nrm004
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© 2007 Oxford University Press

Synthesis, Duplex-forming Ability and Fluorescent Property of OligoDNA Bearing Silylated Fluorophore

Kazuo Shinozuka*, Tohru Sekiguchi, Yumiko Ebara and Tomohisa Moriguchi

Graduate Scholl of Chemistry and Chemical Biology, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu City 376-8515, Japan

* sinozuka{at}chem-bio.gunma-u.ac.jp

Abstract

Novel pyrene derivative conjugated with dimethylsilyl moiety was prepared from bromopyrene. The compound was successfully converted to the corresponding phosphoramidite derivative and incorporated into oligoDNA. In uv-melting study, the resulted fluorescent oligoDNA can discriminate one-base mismatched complement only when the mismatched base is positioned around the middle of the duplex-forming region. On the other hand, the oligoDNA gives marked fluorescence upon binding with the full-matched complement. The intensity of the fluorescent signal of the oligoDNA in a duplex changes depending on the position of the mismatched base of the complement.


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