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Nucleic Acids Symposium Series 2008 52(1):71-72; doi:10.1093/nass/nrn036
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

An efficient fluorescent method for selective detection of mature miRNA species

Yoshio Kato1,*

1Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1, Higashi, Tsukuba, 305-8562, Japan

*Corresponding author. E-mail: y-kato{at}aist.go.jp

Abstract

Methods for specific detection of RNA molecules have been widely developed in biotechnology and diagnostic field. The diagnostic target is recently expanded to non-coding RNA, including microRNAs (miRNAs). miRNAs are initially synthesized as precursor, and then processed to their physiologically active species, miRNAs. Detection of only the active mature miRNA without interference of the precursor forms is extremely important for their functional and physiological significance. We developed a novel fluorescent DNA probe to detect the mature miRNA with high specificity. Since the probe discriminates the mature miRNA from the precursor without electrophoretic separation, it should be useful for real-time detection of the mature miRNAs.


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